Frequently Asked Questions

Quick answers to the questions researchers ask most often

Everything researchers commonly contact us about, organised clearly in one place.

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🛡 Are all products independently tested?

Yes. Every batch is independently verified through Janoshik Analytical HPLC testing.

Each product supplied undergoes analysis by Janoshik Analytical, a Czech laboratory specialising in peptide and compound testing that operates independently from us. Reported purity levels consistently exceed 99%.

When a Janoshik Certificate of Analysis (COA) has been issued for the current batch, a printed copy is included with the order. If a report is not available, the corresponding verification record is published on our Purity page.

Every COA includes a unique verification key that can be checked through Janoshik’s own verification platform, allowing researchers to independently confirm the analytical results.

We also provide information on how to interpret Janoshik HPLC reports for full transparency.

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💧 BAC water + powder reconstitution instructions

Follow these four simple preparation steps:

1. Clean the vial

Use an alcohol prep wipe from the supplied kit to disinfect the vial tops. Allow them to dry fully before use.

2. Draw bacteriostatic water

Using an insulin syringe, withdraw 2 mL of BAC water through the rubber stopper of the vial.

3. Add BAC water carefully

Inject the liquid slowly down the inside wall of the vial. Avoid directing the stream directly onto the lyophilised powder.

4. Dissolve gently

Carefully swirl the vial until the contents are fully dissolved. Do not shake. Allow approximately 30 seconds for complete dissolution before refrigeration.

Use the reconstitution card inside the box lid as a quick reference for future preparation.

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💉 Pen instructions: needle setup, priming and dose dial

Attach a fresh needle

Clean the pen tip using an alcohol wipe, then securely attach a new foil-wrapped needle.

Prime the pen

Turn the dial one click, hold the pen upright and press the dose button. A small droplet at the needle tip confirms successful priming and removal of air.

Select the research setting

Each click delivers a fixed increment according to the pen calibration. Typical resolution is 0.5–1 mg per click, with the dose window displaying the selected amount.

Example: a 6 mg research setting may require approximately 6–12 clicks depending on calibration.

Research administration route

Published research protocols describe subcutaneous administration routes, including areas such as the abdomen, thigh and upper arm. Researchers commonly rotate locations throughout study procedures.

After use

Dispose of the needle safely in a sharps container. Replace the pen cap and return the pen to refrigerated storage.

Each pen provides the number of uses specified for the selected format. When the cartridge is empty, replace it while continuing to use the same pen body.

Research use only. The information provided is based on published research protocols and does not constitute medical advice or guidance for human use.

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❄ Storage information

Lyophilised vial

• Stable for 24+ months
• Store at 2–8°C
• Keep sealed and protected from light
• Do not freeze

Reconstituted solution

• Store at 2–8°C
• Keep in the original glass vial
• Avoid shaking
• Discard if cloudy, discoloured or visibly altered
• Typical storage period: approximately 30 days

BAC water

After opening, refrigerate and use within approximately 30 days.

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📍 Administration information from published research

Published research literature involving NAD+ describes subcutaneous administration routes in pharmacokinetic and clinical research settings, including areas such as the abdomen, thigh and upper arm.

Researchers commonly rotate administration locations during studies to minimise repeated local tissue stress.

This information is provided solely for scientific context from peer-reviewed literature. It should not be interpreted as dosing instructions, medical guidance or recommendations for human use.

Reference: Yoshino et al. 2018

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🎯 Which research protocol should I follow?

The recommended approach is to follow the relevant published research protocol.

Research involving NAD+ commonly uses staged progression, beginning with lower research levels and increasing according to predefined study schedules. The highlighted phase within the protocol materials represents the stage where many research groups continue ongoing investigation.

Researchers new to this compound typically review the initial phase before progressing through later stages according to their study design.

Researchers with previous experience can review the available phase information to determine expected product requirements, duration and format.

For customised research schedules, contact our team before ordering. We can help identify the most suitable option based on your planned research requirements.

Research use only. Information is derived from peer-reviewed literature and provided for scientific context only. It is not medical or human-use guidance.

Reference: Yoshino et al. 2018

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Key Information

Category	Details
Compound	NAD+
Research Category	Longevity Research
Available Sizes	100 mg, 500 mg, 1000 mg
Pricing	From £64
Format	Lyophilised powder in sealed vial and pre-reconstituted pen
Purity	>99% HPLC verified by Janoshik Analytical
Intended Use	In-vitro laboratory research only. Not for human consumption

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Chemical & Reference Data

Information below is compiled from public scientific databases, including PubChem and UniProt, alongside peer-reviewed literature. It is intended for laboratory reference and characterisation purposes only.

Property	Information
Compound Class	Nicotinamide adenine dinucleotide (cellular coenzyme)
Molecular Formula	C21H27N7O14P2
Molecular Weight	663.43 g/mol
CAS Registry Number	53-84-9
PubChem CID	925
Mechanism	Coenzyme involved in redox reactions; substrate for sirtuins, PARPs and NAD+ kinases
Reported Half-life	Short serum half-life reported; intracellular NAD+ dynamics widely studied
Solubility	Suitable for laboratory reconstitution using bacteriostatic water
Storage (Lyophilised)	2–8°C; stable for 18+ months; light sensitive
Storage (Reconstituted)	2–8°C; protect from light
Research Status	Endogenous metabolite widely studied in research

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Research Profile

• Cellular redox coenzyme
• Sirtuin-related research compound
• Investigated in mitochondrial research
• Independently purity tested
• Research use only

NAD+ (Nicotinamide Adenine Dinucleotide) is a naturally occurring cellular coenzyme described in scientific literature for its involvement in redox reactions, sirtuin activity and mitochondrial-related pathways in experimental models.

It is supplied as a lyophilised powder intended exclusively for in-vitro laboratory research.

The information above summarises scientific characterisation from published literature and does not represent claims regarding effects in humans.

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Laboratory Handling

NAD+ is supplied as a freeze-dried powder in a sealed glass vial.

For laboratory preparation, add the required volume of diluent slowly along the inside wall of the vial. Gently swirl until fully dissolved. Avoid shaking or vortex mixing.

Prepared solutions should be stored at 2–8°C and protected from light.

Use the Reconstitution Volume Calculator to determine the resulting concentration in mg/mL.

State	Temperature	Typical Shelf Life
Lyophilised	2–8°C or -20°C	Up to 24 months
Reconstituted	2–8°C	Approximately 4–6 weeks

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Purity & Verification

Every batch of NAD+ supplied undergoes independent HPLC verification by Janoshik Analytical.

Where a Janoshik report is available for the current batch, it is supplied with the order and includes a unique verification key that can be authenticated against Janoshik’s records.

Reported purity consistently exceeds 99%.

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Related Research Compounds

Researchers studying NAD+ commonly investigate related longevity-focused compounds, including:

• GHK-Cu – Longevity Research
• Epithalon – Longevity Research
• MOTS-c – Longevity Research

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Compare Related Compounds

Compare NAD+ with related compounds for differences in mechanism, pricing and laboratory handling considerations.