Frequently Asked Questions

Everything researchers ask us most—answered in one place.

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🛡 Do you test your products?

Yes. Every batch is independently verified using HPLC analysis by Janoshik Analytical.

Every batch of Methylcobalamin (B12) is tested by Janoshik Analytical, an independent Czech peptide and analytical laboratory. Batch purity is consistently reported at greater than 99%.

Where available, a printed Certificate of Analysis (COA) is included with your order. If the current batch report is still pending, the verification record can be viewed on our Purity page.

Each COA includes a unique verification code that can be validated directly through Janoshik’s verification portal, providing independent confirmation of the analytical results.

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💧 Reconstituting BAC Water & Lyophilised Powder

Step 1

Clean the vial stopper using an alcohol prep wipe and allow it to dry.

Step 2

Draw 2 mL of bacteriostatic (BAC) water into an insulin syringe.

Step 3

Slowly inject the BAC water down the inside wall of the vial. Avoid directing the stream onto the powder.

Step 4

Gently swirl until the powder has completely dissolved. Do not shake. Refrigerate immediately after reconstitution.

A reconstitution guide is included inside the product packaging for future reference.

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💉 Using the Research Pen

Attach a New Needle

Clean the pen tip with an alcohol wipe before attaching a fresh sterile needle.

Prime the Pen

Turn the dose dial one click, hold the pen upright and press the dose button until a small drop appears at the needle tip.

Select Your Research Dose

Each click delivers a fixed dosing increment according to the pen’s calibration. Set the required dose according to your research protocol.

Administration Route

Published research involving methylcobalamin has commonly utilised subcutaneous administration, typically in the abdomen, thigh or upper arm.

After Use

Remove and safely dispose of the needle in an approved sharps container. Replace the pen cap and refrigerate.

One refill cartridge provides the number of doses specified for your selected pen size.

Research use only. Information is provided for laboratory reference and is based on published scientific literature. It is not intended as medical advice.

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❄ Storage Guidelines

Lyophilised Powder

• Store at 2–8°C
• Keep sealed and protected from light
• Stable for up to 24 months
• Do not freeze

After Reconstitution

• Refrigerate at 2–8°C
• Protect from light
• Use within approximately 30 days
• Do not shake
• Discard if the solution becomes cloudy or discoloured

BAC Water

Once opened, refrigerate and use within 30 days.

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📍 Administration in Published Research

Published pharmacokinetic studies involving Methylcobalamin (B12) have utilised subcutaneous administration, typically in the abdomen, thigh and upper arm. Research protocols generally rotate injection sites to minimise local tissue irritation.

This information is included for scientific context only and is drawn from published peer-reviewed literature. We do not provide dosing recommendations, administration instructions or medical guidance.

Reference: Watanabe et al. (2007)

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🎯 What Dose Should I Use?

The appropriate dose depends entirely on your research protocol.

Published literature involving methylcobalamin typically follows predefined study protocols. If your project follows an established protocol, we recommend selecting the product size that best aligns with your planned schedule.

If you’re unsure which vial or pen size best suits your research, contact our team before ordering and we’ll help you choose the most suitable option.

Research use only. Information is provided for laboratory research purposes and should not be interpreted as medical or human-use guidance.

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Key Information

Specification	Details
Compound	Methylcobalamin (Vitamin B12)
Research Category	Cofactor Research
Available Size	10 mg
Format	Lyophilised powder in sealed glass vial
Purity	>99% (HPLC verified by Janoshik Analytical)
Intended Use	In-vitro laboratory research only. Not for human consumption.

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Chemical & Reference Data

Property	Details
Compound Class	Methylated form of Vitamin B12
Molecular Formula	C₆₃H₉₁CoN₁₃O₁₄P
Molecular Weight	1344.4 g/mol
CAS Number	13422-55-4
PubChem CID	6436231
Mechanism	Methyl donor cofactor for methionine synthase
Reported Half-Life	Approximately 6 days (human serum)
Solubility	Reconstitutes in bacteriostatic water for laboratory use
Lyophilised Storage	Store at 2–8°C. Protect from light in an amber vial.
Reconstituted Storage	Store at 2–8°C and use within approximately 30 days. Protect from light.
Research Status	Naturally occurring cofactor widely used in biochemical research

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Research Profile

• Bioactive form of Vitamin B12
• Methionine synthase cofactor
• Methyl donor compound
• Independently HPLC verified
• Research use only

Methylcobalamin is the biologically active methylated form of vitamin B12 and functions as an essential cofactor for methionine synthase. It is supplied as a lyophilised powder for in-vitro laboratory research only.

Descriptions are based on published scientific literature and are provided solely for research characterisation. They should not be interpreted as claims regarding therapeutic or clinical effects.

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Laboratory Handling

Methylcobalamin is supplied as a sterile lyophilised powder in a sealed glass vial.

For laboratory reconstitution, slowly add the required volume of bacteriostatic water down the inside wall of the vial. Gently swirl until fully dissolved. Do not shake or vortex.

Store reconstituted solutions at 2–8°C, protected from light, and use within 4–6 weeks, depending on your laboratory protocol.

State	Storage Temperature	Typical Shelf Life
Lyophilised	2–8°C or -20°C	Up to 24 months
Reconstituted	2–8°C	Approximately 4–6 weeks

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Purity & Verification

Every batch of Methylcobalamin (B12) is independently analysed by Janoshik Analytical using HPLC testing.

Where available, each order includes a Certificate of Analysis containing a unique verification code that can be confirmed directly through Janoshik’s verification system.

Reported batch purity is consistently greater than 99%.

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Related Research Compounds

Researchers studying Methylcobalamin (B12) commonly work alongside:

• NAD+
• Glutathione