Frequently Asked Questions

Find answers to the questions researchers ask us most often.

🛡 Is every product tested?

Yes. Every batch undergoes independent purity verification.

Each batch is analysed by Janoshik Analytical, an independent peptide testing laboratory based in the Czech Republic. The lab has no affiliation with us and verifies product purity using HPLC analysis.

Our batches consistently achieve greater than 99% purity.

Where available, the current batch’s Certificate of Analysis (COA) is included with your order. If a printed certificate is unavailable, the latest verification can be viewed on our Purity page.

Every certificate contains a unique verification code that can be confirmed directly through Janoshik’s online verification portal, providing independent confirmation of authenticity.

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💧 Reconstituting Your Peptide in 4 Simple Steps

1. Clean the vial stopper using one of the supplied alcohol wipes and allow it to dry completely.
2. Draw 2 mL of bacteriostatic water into an insulin syringe.
3. Slowly inject the water down the inner wall of the peptide vial, avoiding direct contact with the powder.
4. Gently swirl the vial until the solution becomes clear. Do not shake. Allow approximately 30 seconds for complete dissolution before refrigerating.

The reconstitution guide printed inside the box lid can be used as a quick reference for future use.

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💉 Using the Pen

1. Fit a New Needle

Disinfect the pen tip with an alcohol wipe before attaching a fresh sterile needle.

2. Prime the Pen

Turn the dial one click, point the pen upward, and press the button until a small droplet appears at the needle tip. This confirms the pen is primed and air has been removed.

3. Select Your Research Dose

Rotate the dial until the desired dose is displayed.

Each click corresponds to a fixed delivery amount depending on the pen calibration, typically between 0.5 mg and 1 mg per click.

4. Administer According to Published Research

Published protocols commonly describe subcutaneous administration into the abdomen, thigh, or upper arm.

Hold the pen in place for approximately six seconds before withdrawing.

Dispose of the used needle safely, replace the cap, and store the pen refrigerated.

A single pen provides the number of doses indicated on the protocol cards for your selected size. Replacement cartridges can be inserted into the same pen body when empty.

For research use only. Information reflects published research procedures and is not intended as medical guidance.

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❄ Storage Recommendations

Lyophilised Powder

• Store sealed at 2–8°C
• Protect from light
• Stable for 24 months or longer
• Do not freeze

Reconstituted Solution

• Store between 2–8°C
• Use within approximately 30 days
• Keep in the original vial
• Do not shake
• Discard if the solution becomes cloudy or changes colour

Bacteriostatic Water

• Refrigerate after opening
• Use within 30 days

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📍 Administration Route Used in Published Studies

Published pharmacokinetic and Phase II/III research involving Semaglutide commonly utilised subcutaneous administration in the abdomen, thigh, or upper arm.

Injection sites are typically rotated to minimise local tissue irritation.

Research use only. This information summarises published scientific literature and does not constitute dosing or medical advice.

Reference: Wilding et al., NEJM (2021).

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🎯 What Dose Should Be Used?

Published Semaglutide research generally follows a gradual dose-escalation (titration) schedule.

Researchers begin with the lowest protocol phase before increasing at predefined weekly intervals. This approach is widely used in the literature to reduce gastrointestinal side effects.

Researchers already familiar with the compound can identify the appropriate protocol phase using the dosing guide above.

If your study requires a customised research schedule, contact us before ordering and we’ll help determine the most appropriate vial or pen size.

Research use only. Information reflects published research protocols and should not be interpreted as medical advice.

Reference: Wilding et al., NEJM (2021).

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Key Product Information

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Chemical & Reference Information

The following information is compiled from publicly available scientific databases and peer-reviewed publications for laboratory reference.

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Research Overview

Semaglutide is a 31-amino-acid synthetic peptide that acts as a GLP-1 receptor agonist. Scientific literature reports approximately 94% sequence homology with naturally occurring human GLP-1.

The product is supplied as a lyophilised powder intended exclusively for in-vitro laboratory research.

Descriptions above reflect published scientific literature and are not claims regarding human therapeutic effects.

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Laboratory Handling

Semaglutide is supplied as a freeze-dried powder in a sealed glass vial.

For laboratory preparation:

• Add bacteriostatic water slowly down the inside wall of the vial.
• Avoid directing liquid onto the powder.
• Gently swirl until fully dissolved.
• Do not shake or vortex.
• Store the prepared solution between 2–8°C.
• Use within 4–6 weeks.

A reconstitution calculator can be used to determine the resulting concentration (mg/mL).

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Purity Verification

Every Semaglutide batch is independently tested by Janoshik Analytical using HPLC analysis.

Where available, a Certificate of Analysis accompanies each order and includes a unique verification code that can be validated directly with Janoshik.

Reported purity consistently exceeds 99%.

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Related Research Compounds

Researchers studying Semaglutide frequently also investigate:

• Tirzepatide
• Liraglutide
• Mazdutide

Comparison guides are available for:

• Semaglutide vs Liraglutide
• Semaglutide vs Tirzepatide

These comparisons cover mechanism of action, laboratory handling, and pricing.

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Selected Scientific References

• Lau J. et al. (2015) – Discovery of the once-weekly GLP-1 analogue Semaglutide (PMID: 26117859)
• Wilding J.P.H. et al. (2021) – STEP 1 Trial: Once-weekly Semaglutide in adults with overweight or obesity (PMID: 33567185)